Windows的答案： 我了解到，由于无限运行的动画，无法执行该按钮定义的动作。 （缺乏英语）

在这种情况下，您可以考虑一种在单独线程中进行动画的方法。 并设置相同的变量名称和函数名称使调试变得困难。

这是带有一些随机编辑的代码。 我想给出一个详细的解释，想知道为什么原始代码中有一个黄色按钮，但我不擅长英语，所以我只关注这个问题。

import time
from tkinter import *
import tkinter as tk
import turtle
import random
from threading import Thread


class Application(Frame):
    def __init__(self, master):
        super(Application, self).__init__(master)
        self.switcher = True
        self.speedx = 5
        self.speedy = 5
        self.position_x = 0
        self.position_y = 0
        self.red_clicks_dva = 0
        self.green_clicks_dva = 0
        self.acuity_list_dva = [60, 36, 24, 18, 12, 9, 6, 5, 4, 3, 2.5, 2, 1.5, 1]
        self.acuity_list_4m_dva = [40, 24, 16, 12, 8, 6, 4, 3.33, 2.67, 2, 1.67, 1.33, 1, 0.67]
        self.characters_dva = ['draw_c', 'draw_z', 'draw_o', 'draw_t', 'draw_p', 'draw_f', 'draw_e', 'draw_h', 'draw_v',
                               'draw_d', 'draw_s', 'draw_n', 'draw_r']
        self.width_value = my_window.winfo_screenwidth()
        self.height_value = my_window.winfo_screenheight()
        self.launch_dva()

    def launch_dva(self):
        for item in my_window.winfo_children():
            item.destroy()
        position = self.green_clicks_dva
        selected_characters_dva = random.sample(self.characters_dva, 1)
        print(selected_characters_dva)
        width = self.acuity_list_4m_dva[position] * 0.00145 * 100
        self.width_pixel_dva = (width * 96) / 2.54

        self.canvas1 = tk.Canvas(my_window, height=self.width_pixel_dva + 10, width=self.width_pixel_dva + 10,
                                 bg='white', highlightthickness=0)
        self.canvas1.pack_propagate(False)
        self.canvas1.pack()

        self.canvas1.place(x=self.position_x, y=self.position_y)

        self.canvas2 = tk.Canvas(my_window, height=self.width_pixel_dva + 10, width=self.width_pixel_dva + 10,
                                 bg='white', highlightthickness=0)
        self.canvas2.pack_propagate(False)
        self.canvas2.pack()
        self.canvas2.place(x=self.position_x, y=self.position_y)

        turtle_name = turtle.RawTurtle(self.canvas1)
        turtle_name.speed('fastest')
        turtle_name.color("black", "white")
        self.draw_c(turtle_name, self.width_pixel_dva, 0)

        turtle_name2 = turtle.RawTurtle(self.canvas2)
        turtle_name2.speed('fastest')
        turtle_name2.color("black", "white")
        self.draw_c(turtle_name2, self.width_pixel_dva, 0)

        self.greenbutton_dva = PhotoImage(file='/home/vevek/Applications/RoundGreen.png')
        self.yellowbutton_dva = PhotoImage(file='/home/vevek/Applications/RoundYellow.png')
        self.redbutton_dva = PhotoImage(file='/home/vevek/Applications/Resized red.png')

        self.yellow_dva = Button(my_window, image=self.yellowbutton_dva, borderwidth=0, bg="white",
                                 highlightthickness=0)
        self.yellow_dva.pack(pady=10)
        self.yellow_dva.place(x=width_value / 2, y=height_value - height_value / 4, anchor="center")

        self.green_dva = Button(my_window, image=self.greenbutton_dva, bg="white", command=self.greenbutton_dva_clicked,
                                highlightthickness=0, borderwidth=0)
        self.green_dva.pack(padx=10)
        self.green_dva.place(x=(width_value / 2) - 100, y=height_value - height_value / 4, anchor="center")

        self.red_dva = Button(my_window, image=self.redbutton_dva, bg="white", command=self.redbutton_dva_clicked,
                              highlightthickness=0, borderwidth=0)
        self.red_dva.pack(padx=10)
        self.red_dva.place(x=(width_value / 2) + 100, y=height_value - height_value / 4, anchor="center")

        self.move_canvas()
        self.t1 = Thread(target=self.switch_canvas)
        self.t1.start()
        # my_window.after(0, self.switch_canvas)

    def move_canvas(self):
        self.position_x += self.speedx
        self.position_y += self.speedy
        if (self.switcher):
            self.canvas1.place(x=self.position_x, y=self.position_y)
        else:
            self.canvas2.place(x=self.position_x, y=self.position_y)
        my_window.update()
        if self.position_x + self.width_pixel_dva >= self.width_value or self.position_x <= 0:
            self.speedx *= -1
        if self.position_y + self.width_pixel_dva >= (3 * self.height_value / 4) - 100 or self.position_y <= 0:
            self.speedy *= -1

    def switch_canvas(self):
        if (self.switcher):
            self.canvas2.pack_forget()
            self.move_canvas()
        else:
            self.canvas1.pack_forget()
            self.move_canvas()
        self.switcher = not self.switcher

        my_window.after(10, self.switch_canvas)

    def greenbutton_dva_clicked(self):
        self.green_clicks_dva += 1
        print("green button clicked")
        # self.launch_dva()

    def redbutton_dva_clicked(self):
        print("Your dynamic visual acuity is " + str(self.acuity_list_dva[self.green_clicks_dva]))
        self.dva_done_flag = 1
        # self.test_page()

    def draw_c(self, turtle, width, shade):
        turtle.penup()
        turtle.setposition(((width / 2) - (width / 5)), -((width / 4)))
        turtle.pendown()
        turtle.shape("blank")

        turtle.pensize(width / 5)
        turtle.pencolor(shade, shade, shade)

        turtle.left(50)
        turtle.circle((width / 2 - width / 10), -280)
        turtle.penup()
        return turtle.turtlesize()


my_window = tk.Tk()
width_value = my_window.winfo_screenwidth()
height_value = my_window.winfo_screenheight()
my_window.geometry("%dx%d+0+0" % (width_value, height_value))
my_window.configure(bg='white')
my_window.title('EYIRIS')

app = Application(my_window)

my_window.mainloop()

希望这可以帮助。